IL-24 CLIA Kit

Catalog No. ABIN5655919

Product Details IL-24 CLIA Kit

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 24 (IL24). No significant cross-reactivity or interference between Interleukin 24 (IL24) and analogues was observed.

Sensitivity: 0.65 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 24 (IL24). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 24 (IL24) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 24 (IL24) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: IL-24 (IL24) (Interleukin 24 (IL24))

Alternative Name: Interleukin 24 (IL24 Products)

Synonyms: C49A CLIA Kit, FISP CLIA Kit, IL10B CLIA Kit, MDA7 CLIA Kit, MOB5 CLIA Kit, ST16 CLIA Kit, Mda7 CLIA Kit, St16 CLIA Kit, Mda-7 CLIA Kit, IL24 CLIA Kit, interleukin 24 CLIA Kit, IL24 CLIA Kit, Il24 CLIA Kit

Background:

Gene Name: Interleukin 24

Gene Aliases: C49A, FISP, IL10B, MDA7, Mob-5, ST16, Mda-7, Melanoma Differentiation Association Protein 7, Suppression Of Tumorigenicity 16

Gene ID: 170819

UniProt: Q9JI24

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Autophagy