KLF4 ELISA Kit

Catalog No. ABIN5656257

Product Details KLF4 ELISA Kit

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Kruppel Like Factor 4, Gut (KLF4). No significant cross-reactivity or interference between Kruppel Like Factor 4, Gut (KLF4) and analogues was observed.

Sensitivity: 0.058 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kruppel Like Factor 4, Gut (KLF4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kruppel Like Factor 4, Gut (KLF4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kruppel Like Factor 4, Gut (KLF4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kruppel Like Factor 4, Gut (KLF4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kruppel Like Factor 4, Gut (KLF4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for KLF4

Target: KLF4 (Kruppel-Like Factor 4 (Gut) (KLF4))

Alternative Name: Kruppel Like Factor 4, Gut (KLF4 Products)

Synonyms: EZF ELISA Kit, GKLF ELISA Kit, Gklf ELISA Kit, Zie ELISA Kit, KLF4 ELISA Kit, biklf ELISA Kit, biklf-A ELISA Kit, Kruppel like factor 4 ELISA Kit, Kruppel-like factor 4 (gut) ELISA Kit, Kruppel-like factor 4 ELISA Kit, Kruppel-like factor 4 (gut) L homeolog ELISA Kit, KLF4 ELISA Kit, Klf4 ELISA Kit, klf4 ELISA Kit, klf4.L ELISA Kit

Background:

Gene Name: Kruppel Like Factor 4, Gut

Gene Aliases: EZF, GKLF, Gut-Enriched Krüppel-Like Factor, Epithelial zinc finger protein EZF

Gene ID: 9314

UniProt: O43474

Pathways: Peptide Hormone Metabolism, Stem Cell Maintenance