PSMB8 ELISA Kit

Catalog No. ABIN5656323

Product Details PSMB8 ELISA Kit

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Large Multifunctional Peptidase 7 (LMP7). No significant cross-reactivity or interference between Large Multifunctional Peptidase 7 (LMP7) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Large Multifunctional Peptidase 7 (LMP7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Large Multifunctional Peptidase 7 (LMP7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Large Multifunctional Peptidase 7 (LMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Large Multifunctional Peptidase 7 (LMP7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Large Multifunctional Peptidase 7 (LMP7) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PSMB8

Target: PSMB8 (Proteasome (Prosome, Macropain) Subunit, beta Type, 8 (Large Multifunctional Peptidase 7) (PSMB8))

Alternative Name: Large Multifunctional Peptidase 7 (PSMB8 Products)

Synonyms: ALDD ELISA Kit, D6S216 ELISA Kit, D6S216E ELISA Kit, JMP ELISA Kit, LMP7 ELISA Kit, NKJO ELISA Kit, PSMB5i ELISA Kit, RING10 ELISA Kit, ATP6V0D2 ELISA Kit, lmp7 ELISA Kit, prosome ELISA Kit, macropain ELISA Kit, Lmp-7 ELISA Kit, Lmp7 ELISA Kit, Ring10 ELISA Kit, proteasome subunit beta 8 ELISA Kit, proteasome subunit beta 8A ELISA Kit, proteasome (prosome, macropain) subunit, beta type 8 (large multifunctional peptidase 7) ELISA Kit, PSMB8 ELISA Kit, psmb8a ELISA Kit, Psmb8 ELISA Kit

Background:

Gene Name: Large Multifunctional Peptidase 7

Gene Aliases: PSMB8, RING10, PSMB5i, Beta5i, Proteasome(Prosome,Macropain)Subunit,Beta Type 8, Multicatalytic endopeptidase complex subunit C13, Really interesting new gene 10 protein

Gene ID: 5696

UniProt: P28062

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA