LTBP1 ELISA Kit

Catalog No. ABIN5656325

Product Details LTBP1 ELISA Kit

Target: LTBP1 (Latent Transforming Growth Factor beta Binding Protein 1 (LTBP1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). No significant cross-reactivity or interference between Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) and analogues was observed.

Sensitivity: 0.053 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for LTBP1

Target: LTBP1 (Latent Transforming Growth Factor beta Binding Protein 1 (LTBP1))

Alternative Name: Latent Transforming Growth Factor Beta Binding Protein 1 (LTBP1 Products)

Synonyms: ltgf-1 ELISA Kit, LTBP1 ELISA Kit, 9430031G15Rik ELISA Kit, 9830146M04 ELISA Kit, Ltbp-1 ELISA Kit, Tgfb ELISA Kit, b2b1000Clo ELISA Kit, latent transforming growth factor beta binding protein 1 S homeolog ELISA Kit, latent transforming growth factor beta binding protein 1 ELISA Kit, latent transforming growth factor beta binding protein 2 ELISA Kit, latent TGFbeta binding protein ELISA Kit, latent-transforming growth factor beta-binding protein 1 ELISA Kit, ltbp1.S ELISA Kit, ltbp1 ELISA Kit, LTBP1 ELISA Kit, LTBP2 ELISA Kit, LOC100542113 ELISA Kit, Ltbp1 ELISA Kit

Background:

Gene Name: Latent Transforming Growth Factor Beta Binding Protein 1

Gene Aliases: TGF-beta1-BP-1, Transforming growth factor beta-1-binding protein 1

Gene ID: 268977

UniProt: Q8CG19

Pathways: Maintenance of Protein Location, Growth Factor Binding