LCAT CLIA Kit

Catalog No. ABIN5656328

Product Details LCAT CLIA Kit

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 2.34 ng/mL - 150 ng/mL

Minimum Detection Limit: 2.34 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Lecithin Cholesterol Acyltransferase (LCAT). No significant cross-reactivity or interference between Lecithin Cholesterol Acyltransferase (LCAT) and analogues was observed.

Sensitivity: 0.1 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lecithin Cholesterol Acyltransferase (LCAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lecithin Cholesterol Acyltransferase (LCAT) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lecithin Cholesterol Acyltransferase (LCAT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: Lecithin Cholesterol Acyltransferase (LCAT Products)

Synonyms: AI046659 CLIA Kit, D8Wsu61e CLIA Kit, MGC82035 CLIA Kit, lcat CLIA Kit, MGC88964 CLIA Kit, LCAT CLIA Kit, lecithin-cholesterol acyltransferase CLIA Kit, lecithin cholesterol acyltransferase CLIA Kit, lecithin-cholesterol acyltransferase L homeolog CLIA Kit, solute carrier family 12 member 4 CLIA Kit, fragile site, aphidicolin type, common, fra(13)(q13.2) CLIA Kit, LCAT CLIA Kit, Lcat CLIA Kit, lcat.L CLIA Kit, lcat CLIA Kit, SLC12A4 CLIA Kit, FRA13A CLIA Kit

Background:

Gene Name: Lecithin Cholesterol Acyltransferase

Gene Aliases: phosphatidylcholine-sterol O-acyltransferase, Phospholipid-cholesterol acyltransferase

Gene ID: 3931

UniProt: P04180

Pathways: Lipid Metabolism