NR5A2 + LRH1 ELISA Kit

Catalog No. ABIN5656473

Product Details NR5A2 + LRH1 ELISA Kit

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Liver Receptor Homolog 1 (LRH1). No significant cross-reactivity or interference between Liver Receptor Homolog 1 (LRH1) and analogues was observed.

Sensitivity: 0.063 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1 (LRH1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Liver Receptor Homolog 1 (LRH1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Liver Receptor Homolog 1 (LRH1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Liver Receptor Homolog 1 (LRH1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Liver Receptor Homolog 1 (LRH1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for NR5A2 + LRH1

Target: NR5A2 + LRH1 (NR5A2) (Nuclear Receptor Subfamily 5, Group A, Member 2 (NR5A2))

Alternative Name: Liver Receptor Homolog 1 (NR5A2 Products)

Synonyms: xff1rashort ELISA Kit, NR5A2 ELISA Kit, b1f ELISA Kit, b1f2 ELISA Kit, cpf ELISA Kit, ff1r ELISA Kit, ftf ELISA Kit, ftz-f1 ELISA Kit, ftz-f1beta ELISA Kit, hb1f ELISA Kit, hb1f-2 ELISA Kit, lrh-1 ELISA Kit, lrh1 ELISA Kit, xff1ra ELISA Kit, B1F ELISA Kit, B1F2 ELISA Kit, CPF ELISA Kit, FTF ELISA Kit, FTZ-F1 ELISA Kit, FTZ-F1beta ELISA Kit, LRH-1 ELISA Kit, LRH1 ELISA Kit, hB1F-2 ELISA Kit, xFF1rB ELISA Kit, AU020803 ELISA Kit, D1Ertd308e ELISA Kit, Ftf ELISA Kit, UF2-H3B ELISA Kit, Lrh-1 ELISA Kit, nuclear receptor subfamily 5 group A member 2 S homeolog ELISA Kit, nuclear receptor subfamily 5 group A member 2 ELISA Kit, nuclear receptor subfamily 5 group A member 2 L homeolog ELISA Kit, nuclear receptor subfamily 5, group A, member 2 ELISA Kit, nr5a2.S ELISA Kit, NR5A2 ELISA Kit, nr5a2 ELISA Kit, nr5a2.L ELISA Kit, Nr5a2 ELISA Kit

Background:

Gene Name: Liver Receptor Homolog 1

Gene Aliases: NR5A2, B1F, B1F2, CPF, FTF, FTZ-F1, LRH-1, hB1F, hB1F-2, Hepatocytic transcription factor, Alpha-1-fetoprotein transcription factor, Nuclear Receptor Subfamily 5,Group A,Member 2

Gene ID: 60349

UniProt: Q9QWM1

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway