CCL24 CLIA Kit

Catalog No. ABIN5657052

Product Details CCL24 CLIA Kit

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 7.81 pg/mL - 2000 pg/mL

Minimum Detection Limit: 7.81 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Myeloid Progenitor Inhibitory Factor 2 (MPIF2). No significant cross-reactivity or interference between Myeloid Progenitor Inhibitory Factor 2 (MPIF2) and analogues was observed.

Sensitivity: 0.97 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Myeloid Progenitor Inhibitory Factor 2 (MPIF2) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myeloid Progenitor Inhibitory Factor 2 (MPIF2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: CCL24 (Chemokine (C-C Motif) Ligand 24 (CCL24))

Alternative Name: Myeloid Progenitor Inhibitory Factor 2 (CCL24 Products)

Synonyms: Ckb-6 CLIA Kit, MPIF-2 CLIA Kit, MPIF2 CLIA Kit, SCYA24 CLIA Kit, CKb-6 CLIA Kit, Scya24 CLIA Kit, C-C motif chemokine ligand 24 CLIA Kit, chemokine (C-C motif) ligand 24 CLIA Kit, CCL24 CLIA Kit, Ccl24 CLIA Kit

Background:

Gene Name: Myeloid Progenitor Inhibitory Factor 2

Gene Aliases: CCL24, SCYA24, Ckb-6, Eotaxin-2, Chemokine C-C-Motif Ligand 24, Eosinophil chemotactic protein 2, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 24

Gene ID: 56221

UniProt: Q9JKC0

Pathways: Regulation of Actin Filament Polymerization