Glucocorticoid Receptor ELISA Kit

Catalog No. ABIN5657465

Product Details Glucocorticoid Receptor ELISA Kit

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). No significant cross-reactivity or interference between Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) and analogues was observed.

Sensitivity: 0.054 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Glucocorticoid Receptor

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Alternative Name: Nuclear Receptor Subfamily 3, Group C, Member 1 (NR3C1 Products)

Synonyms: GR ELISA Kit, gcr ELISA Kit, grl ELISA Kit, xGR ELISA Kit, gccr ELISA Kit, gr ELISA Kit, zgc:113038 ELISA Kit, NR3C1 ELISA Kit, GCL ELISA Kit, LOC100302444 ELISA Kit, Gcr ELISA Kit, Grl ELISA Kit, Grl-1 ELISA Kit, Grl1 ELISA Kit, GRL ELISA Kit, GCCR ELISA Kit, GCR ELISA Kit, NO ELISA Kit, GR-A ELISA Kit, nr3c1 ELISA Kit, nuclear receptor subfamily 3 group C member 1 ELISA Kit, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) ELISA Kit, nuclear receptor subfamily 3, group C, member 1 ELISA Kit, glucocorticoid receptor ELISA Kit, NR3C1 ELISA Kit, nr3c1 ELISA Kit, LOC100302444 ELISA Kit, Nr3c1 ELISA Kit

Background:

Gene Name: Nuclear Receptor Subfamily 3, Group C, Member 1

Gene Aliases: GR, GCCR, GCR, GR, GRL, NR3-C1, Glucocorticoid Receptor

Gene ID: 24413

UniProt: P06536

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process