PCNT ELISA Kit

Catalog No. ABIN5657696

Product Details PCNT ELISA Kit

Target: PCNT (Pericentrin (PCNT))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pericentrin (PCNT). No significant cross-reactivity or interference between Pericentrin (PCNT) and analogues was observed.

Sensitivity: 0.119 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pericentrin (PCNT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pericentrin (PCNT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pericentrin (PCNT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pericentrin (PCNT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pericentrin (PCNT) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pericentrin (PCNT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PCNT

Target: PCNT (Pericentrin (PCNT))

Alternative Name: Pericentrin (PCNT Products)

Synonyms: AKAP450 ELISA Kit, CG13459 ELISA Kit, CG18648 ELISA Kit, CG33957 ELISA Kit, CG6735 ELISA Kit, CP309 ELISA Kit, Cp309 ELISA Kit, D-PLP ELISA Kit, DPLP ELISA Kit, Dmel\\CG33957 ELISA Kit, Dplp ELISA Kit, PACT ELISA Kit, PLP ELISA Kit, Plp ELISA Kit, d-plp ELISA Kit, dPLP ELISA Kit, dplp ELISA Kit, l(3)s2172 ELISA Kit, pPlp ELISA Kit, KEN ELISA Kit, MOPD2 ELISA Kit, PCN ELISA Kit, PCNT2 ELISA Kit, PCNTB ELISA Kit, PCTN2 ELISA Kit, SCKL4 ELISA Kit, DIP2A ELISA Kit, AW476095 ELISA Kit, C86676 ELISA Kit, Pcnt2 ELISA Kit, kendrin ELISA Kit, m239Asp ELISA Kit, m275Asp ELISA Kit, pericentrin ELISA Kit, Pericentrin-like protein ELISA Kit, pericentrin (kendrin) ELISA Kit, LOC100055203 ELISA Kit, Plp ELISA Kit, PCNT ELISA Kit, pcnt ELISA Kit, Pcnt ELISA Kit

Background:

Gene Name: Pericentrin

Gene Aliases: KEN, PCN, PCNT2, PCNTB, PCTN2, SCKL4, Kendrin, Seckel Syndrome 4, Pericentrin-B

Gene ID: 5116

UniProt: O95613

Pathways: Sensory Perception of Sound, M Phase, SARS-CoV-2 Protein Interactome