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PLA2G7 ELISA Kit

Lp-PLA2 Reactivity: Rat Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5657805
  • Target See all PLA2G7 (Lp-PLA2) ELISA Kits
    PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
    Reactivity
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    12.5 pg/mL - 800 pg/mL
    Minimum Detection Limit
    12.5 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Lipoprotein Associated (LpPLA2). No significant cross-reactivity or interference between Phospholipase A2, Lipoprotein Associated (LpPLA2) and analogues was observed.
    Sensitivity
    5.1 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Lipoprotein Associated (LpPLA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Lipoprotein Associated (LpPLA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Lipoprotein Associated (LpPLA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Phospholipase A2, Lipoprotein Associated (LpPLA2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all PLA2G7 (Lp-PLA2) ELISA Kits
    PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
    Alternative Name
    Phospholipase A2, Lipoprotein Associated (Lp-PLA2 Products)
    Synonyms
    LDL-PLA2 ELISA Kit, LP-PLA2 ELISA Kit, PAFAD ELISA Kit, PAFAH ELISA Kit, Lp-PLA2 ELISA Kit, PAF-AH ELISA Kit, R75400 ELISA Kit, zgc:77563 ELISA Kit, phospholipase A2 group VII ELISA Kit, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) ELISA Kit, PLA2G7 ELISA Kit, Pla2g7 ELISA Kit, pla2g7 ELISA Kit
    Background

    Gene Name: Phospholipase A2, Lipoprotein Associated

    Gene Aliases: PLA2G7, PAF-AH, PAFAH, Lp-PLA2, LDL-PLA2, Platelet Activating Factor Acetylhydrolase,Plasma, Phospholipase A2,Group VII, LDL-associated phospholipase A2

    Pathways
    Peptide Hormone Metabolism
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