Pleiotrophin CLIA Kit

Catalog No. ABIN5657948

Product Details Pleiotrophin CLIA Kit

Target: Pleiotrophin (PTN)

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Pleiotrophin (PTN). No significant cross-reactivity or interference between Pleiotrophin (PTN) and analogues was observed.

Sensitivity: 1.19 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Pleiotrophin (PTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pleiotrophin (PTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pleiotrophin (PTN) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pleiotrophin (PTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Pleiotrophin (PTN)

Alternative Name: Pleiotrophin (PTN Products)

Synonyms: zHBNF CLIA Kit, zgc:111787 CLIA Kit, harp CLIA Kit, hbnf CLIA Kit, X-PTF CLIA Kit, hbgf8 CLIA Kit, negf1 CLIA Kit, ptn CLIA Kit, PTN CLIA Kit, HARP CLIA Kit, HBGF8 CLIA Kit, HBNF CLIA Kit, NEGF1 CLIA Kit, PTN-A CLIA Kit, HB-GAM CLIA Kit, HBBM CLIA Kit, HBGF-8 CLIA Kit, OSF-1 CLIA Kit, HBBN CLIA Kit, OSF CLIA Kit, Osf-1 CLIA Kit, Osf1 CLIA Kit, Hbnf CLIA Kit, pleiotrophin CLIA Kit, pleiotrophin L homeolog CLIA Kit, ptn CLIA Kit, PTN CLIA Kit, ptn.L CLIA Kit, Ptn CLIA Kit

Background:

Gene Name: Pleiotrophin

Gene Aliases: HARP, HBGF8, HBNF, NEGF1, Heparin Binding Growth Factor 8, Neurite Growth-Promoting Factor 1, Heparin Affin Regulatory Peptide, Heparin Binding Growth Associated Molecule

Gene ID: 5764

UniProt: P21246

Pathways: RTK Signaling