PD-L1 CLIA Kit
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- Target See all PD-L1 CLIA Kits
- PD-L1 (CD274 (PD-L1))
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Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 312.5 pg/mL - 20000 pg/mL
- Minimum Detection Limit
- 312.5 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). No significant cross-reactivity or interference between Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) and analogues was observed.
- Sensitivity
- 11.5 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 1 Ligand 1 (PDCD1LG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all PD-L1 CLIA Kits
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- Programmed Cell Death Protein 1 Ligand 1 (PD-L1 Products)
- Synonyms
- B7-H CLIA Kit, B7H1 CLIA Kit, PD-L1 CLIA Kit, PDCD1L1 CLIA Kit, PDCD1LG1 CLIA Kit, PDL1 CLIA Kit, A530045L16Rik CLIA Kit, B7h1 CLIA Kit, Pdcd1l1 CLIA Kit, Pdcd1lg1 CLIA Kit, Pdl1 CLIA Kit, RGD1566211 CLIA Kit, CD274 molecule CLIA Kit, CD274 antigen CLIA Kit, programmed cell death 1 ligand 2 CLIA Kit, CD274 CLIA Kit, Cd274 CLIA Kit, PDCD1LG2 CLIA Kit
- Background
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Gene Name: Programmed Cell Death Protein 1 Ligand 1
Gene Aliases: CD274, PDL1, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1-LG1, Programed Death Ligand 1
- Gene ID
- 29126
- UniProt
- Q9NZQ7
- Pathways
- Cancer Immune Checkpoints
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