F2RL1 CLIA Kit
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- Target See all F2RL1 CLIA Kits
- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
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Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 156.25 pg/mL - 10000 pg/mL
- Minimum Detection Limit
- 156.25 pg/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Protease Activated Receptor 2 (PAR2). No significant cross-reactivity or interference between Protease Activated Receptor 2 (PAR2) and analogues was observed.
- Sensitivity
- 4.9 pg/mL
- Top Product
- Discover our top product F2RL1 ELISA Kit
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 2 (PAR2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 2 (PAR2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Protease Activated Receptor 2 (PAR2) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protease Activated Receptor 2 (PAR2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protease Activated Receptor 2 (PAR2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all F2RL1 CLIA Kits
- F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))
- Alternative Name
- Protease Activated Receptor 2 (F2RL1 Products)
- Synonyms
- par2 CLIA Kit, GPR11 CLIA Kit, PAR2 CLIA Kit, Gpcr11 CLIA Kit, PAR-2 CLIA Kit, Par2 CLIA Kit, Par-2 CLIA Kit, Proteinase-activated receptor 2 CLIA Kit, F2R like trypsin receptor 1 CLIA Kit, coagulation factor II (thrombin) receptor-like 1 CLIA Kit, par2 CLIA Kit, F2RL1 CLIA Kit, F2rl1 CLIA Kit
- Background
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Gene Name: Protease Activated Receptor 2
Gene Aliases: F2RL1, F2-RL1, GPR11, PAR2, Coagulation Factor II(thrombin)receptor-Like 1, G-protein coupled receptor 11, Thrombin receptor-like 1
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome
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