PPP2R4 ELISA Kit

Catalog No. ABIN5658246

Product Details PPP2R4 ELISA Kit

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4). No significant cross-reactivity or interference between Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PPP2R4

Target: PPP2R4 (Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4))

Alternative Name: Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4 Products)

Synonyms: PP2A ELISA Kit, PR53 ELISA Kit, PTPA ELISA Kit, 2610042B21Rik ELISA Kit, C77440 ELISA Kit, N28142 ELISA Kit, PTPRA ELISA Kit, Ppp2r4 ELISA Kit, fc23d07 ELISA Kit, ppp2r4 ELISA Kit, wu:fc23d07 ELISA Kit, zgc:92766 ELISA Kit, pp2a ELISA Kit, ppp2r4.L ELISA Kit, pr53 ELISA Kit, ptpa ELISA Kit, protein phosphatase 2 phosphatase activator ELISA Kit, protein phosphatase 2 protein activator ELISA Kit, protein phosphatase 2 phosphatase activator L homeolog ELISA Kit, PTPA ELISA Kit, Ptpa ELISA Kit, ptpa ELISA Kit, ptpa.L ELISA Kit

Background:

Gene Name: Protein Phosphatase 2A Activator, Regulatory Subunit 4

Gene Aliases: PP2A, PR53, PTPA, Phosphotyrosyl Phosphatase Activator, PP2A Phosphatase Activator, PP2A, subunit B', PR53 isoform, Serine/threonine-protein phosphatase 2A activator

Gene ID: 362102

UniProt: B2RYQ2

Pathways: PI3K-Akt Signaling, M Phase, Hepatitis C, Toll-Like Receptors Cascades