CCL21 CLIA Kit
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- Target See all CCL21 CLIA Kits
- CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
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Reactivity
- Mouse
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 31.25 pg/mL - 2000 pg/mL
- Minimum Detection Limit
- 31.25 pg/mL
- Application
- ELISA
- Sample Type
- Plasma, Serum
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Secondary Lymphoid Tissue Chemokine (SLC). No significant cross-reactivity or interference between Secondary Lymphoid Tissue Chemokine (SLC) and analogues was observed.
- Sensitivity
- 1.31 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Secondary Lymphoid Tissue Chemokine (SLC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Secondary Lymphoid Tissue Chemokine (SLC) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secondary Lymphoid Tissue Chemokine (SLC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all CCL21 CLIA Kits
- CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))
- Alternative Name
- Secondary Lymphoid Tissue Chemokine (CCL21 Products)
- Synonyms
- 6Ckine CLIA Kit, CKb9 CLIA Kit, ECL CLIA Kit, SCYA21 CLIA Kit, SLC CLIA Kit, TCA4 CLIA Kit, ccl21 CLIA Kit, Ccl21b CLIA Kit, C-C motif chemokine ligand 21 CLIA Kit, C-C motif chemokine 21 CLIA Kit, CCL21 CLIA Kit, ccl21 CLIA Kit, Ccl21 CLIA Kit
- Background
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Gene Name: Secondary Lymphoid Tissue Chemokine
Gene Aliases: CCL21, 6Ckine, CKb9, ECL, SCYA21, TCA4, ECL, Chemokine(C-C-Motif)Ligand 21, Beta Chemokine Exodus-2, Efficient Chemoattractant For Lymphocytes
- Gene ID
- 100041504
- UniProt
- P86793
- Pathways
- Regulation of Actin Filament Polymerization
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