SHC1 ELISA Kit

Catalog No. ABIN5658775

Product Details SHC1 ELISA Kit

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of SHC-Transforming Protein 1 (SHC1). No significant cross-reactivity or interference between SHC-Transforming Protein 1 (SHC1) and analogues was observed.

Sensitivity: 0.117 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to SHC-Transforming Protein 1 (SHC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SHC-Transforming Protein 1 (SHC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SHC-Transforming Protein 1 (SHC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SHC-Transforming Protein 1 (SHC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SHC-Transforming Protein 1 (SHC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SHC-Transforming Protein 1 (SHC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Alternative Name: SHC-Transforming Protein 1 (SHC1 Products)

Synonyms: SHC1 ELISA Kit, SHC ELISA Kit, SHCA ELISA Kit, P66shc ELISA Kit, si:dkey-246i14.4 ELISA Kit, wu:fc10h03 ELISA Kit, shc ELISA Kit, p66shc ELISA Kit, Shc ELISA Kit, ShcA ELISA Kit, p66 ELISA Kit, SHC adaptor protein 1 ELISA Kit, Shc1p ELISA Kit, squalene--hopene cyclase ELISA Kit, SHC (Src homology 2 domain containing) transforming protein 1 ELISA Kit, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog ELISA Kit, src homology 2 domain-containing transforming protein C1 ELISA Kit, SHC1 ELISA Kit, shc1 ELISA Kit, Shc1 ELISA Kit, shc1.L ELISA Kit

Background:

Gene Name: SHC-Transforming Protein 1

Gene Aliases: SHC, SHCA, p52SHC, p66, p66SHC, SHC-transforming protein 3, SHC-transforming protein A, Src homology 2 domain-containing-transforming protein C1

Gene ID: 6464

UniProt: P29353

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2