SLC16A3 ELISA Kit

Catalog No. ABIN5658858

Product Details SLC16A3 ELISA Kit

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 16, Member 3 (SLC16A3). No significant cross-reactivity or interference between Solute Carrier Family 16, Member 3 (SLC16A3) and analogues was observed.

Sensitivity: 0.28 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 16, Member 3 (SLC16A3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 16, Member 3 (SLC16A3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 16, Member 3 (SLC16A3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 16, Member 3 (SLC16A3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 16, Member 3 (SLC16A3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 16, Member 3 (SLC16A3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC16A3

Target: SLC16A3 (Solute Carrier Family 16 (Monocarboxylic Acid Transporters), Member 3 (SLC16A3))

Alternative Name: Solute Carrier Family 16, Member 3 (SLC16A3 Products)

Synonyms: MCT 3 ELISA Kit, MCT 4 ELISA Kit, MCT-3 ELISA Kit, MCT-4 ELISA Kit, MCT3 ELISA Kit, MCT4 ELISA Kit, mct ELISA Kit, slc16a3b ELISA Kit, Mct3 ELISA Kit, Mct4 ELISA Kit, MCT4b ELISA Kit, wu:fd20b02 ELISA Kit, wu:fi38f12 ELISA Kit, zgc:55905 ELISA Kit, solute carrier family 16 member 3 ELISA Kit, solute carrier family 16 member 3 L homeolog ELISA Kit, solute carrier family 16 (monocarboxylic acid transporters), member 3 ELISA Kit, solute carrier family 16 (monocarboxylate transporter), member 3 ELISA Kit, SLC16A3 ELISA Kit, slc16a3.L ELISA Kit, Slc16a3 ELISA Kit, slc16a3 ELISA Kit

Background:

Gene Name: Solute Carrier Family 16, Member 3

Gene Aliases: MCT4, Monocarboxylic Acid Transporter 4, Monocarboxylate Transporter 4

Pathways: Warburg Effect