SLC30A8 ELISA Kit

Catalog No. ABIN5658864

Product Details SLC30A8 ELISA Kit

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 30 Member 8 (SLC30A8). No significant cross-reactivity or interference between Solute Carrier Family 30 Member 8 (SLC30A8) and analogues was observed.

Sensitivity: 0.057 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 30 Member 8 (SLC30A8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 30 Member 8 (SLC30A8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 30 Member 8 (SLC30A8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 30 Member 8 (SLC30A8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC30A8

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Alternative Name: Solute Carrier Family 30 Member 8 (SLC30A8 Products)

Synonyms: C820002P14Rik ELISA Kit, ZnT-8 ELISA Kit, ZnT8 ELISA Kit, slc30a3 ELISA Kit, znt8 ELISA Kit, ZNT8 ELISA Kit, E130106K10Rik ELISA Kit, Gm212 ELISA Kit, wu:fe49g03 ELISA Kit, RGD1305098 ELISA Kit, solute carrier family 30 member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 L homeolog ELISA Kit, solute carrier family 30, member 10 ELISA Kit, SLC30A8 ELISA Kit, Slc30a8 ELISA Kit, slc30a8.L ELISA Kit, slc30a8 ELISA Kit, Slc30a10 ELISA Kit

Background:

Gene Name: Solute Carrier Family 30 Member 8

Gene Aliases: ZNT8, Zinc Transporter 8

Gene ID: 169026

UniProt: Q8IWU4

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis