SLC6A19 ELISA Kit

Catalog No. ABIN5658874

Product Details SLC6A19 ELISA Kit

Target: SLC6A19 (Solute Carrier Family 6 (Neutral Amino Acid Transporter), Member 19 (SLC6A19))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Solute Carrier Family 6, Member 19 (SLC6A19). No significant cross-reactivity or interference between Solute Carrier Family 6, Member 19 (SLC6A19) and analogues was observed.

Sensitivity: 0.252 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Solute Carrier Family 6, Member 19 (SLC6A19). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Solute Carrier Family 6, Member 19 (SLC6A19). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Solute Carrier Family 6, Member 19 (SLC6A19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Solute Carrier Family 6, Member 19 (SLC6A19) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Solute Carrier Family 6, Member 19 (SLC6A19) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Solute Carrier Family 6, Member 19 (SLC6A19) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SLC6A19

Target: SLC6A19 (Solute Carrier Family 6 (Neutral Amino Acid Transporter), Member 19 (SLC6A19))

Alternative Name: Solute Carrier Family 6, Member 19 (SLC6A19 Products)

Synonyms: B0AT1 ELISA Kit, HND ELISA Kit, 4632401C08Rik ELISA Kit, B<0>AT1 ELISA Kit, B0at1 ELISA Kit, slc6a19a ELISA Kit, zgc:162095 ELISA Kit, XROSIT ELISA Kit, slc6a19 ELISA Kit, wu:fd58f10 ELISA Kit, zgc:66050 ELISA Kit, solute carrier family 6 member 19 ELISA Kit, solute carrier family 6 (neurotransmitter transporter), member 19 ELISA Kit, solute carrier family 6 (neutral amino acid transporter), member 19a, tandem duplicate 1 ELISA Kit, solute carrier family 6 (neutral amino acid transporter), member 19 S homeolog ELISA Kit, solute carrier family 6 (neutral amino acid transporter), member 19b ELISA Kit, SLC6A19 ELISA Kit, Slc6a19 ELISA Kit, slc6a19a.1 ELISA Kit, slc6a19.S ELISA Kit, slc6a19b ELISA Kit

Background:

Gene Name: Solute Carrier Family 6, Member 19

Gene Aliases: B0AT1, HND, Sodium-Dependent Neutral Amino Acid Transporter B(0)AT1, Hartnup Disease, System B(0) neutral amino acid transporter AT1