TCIRG1 ELISA Kit

Catalog No. ABIN5659081

Product Details TCIRG1 ELISA Kit

Target: TCIRG1 (T-Cell, Immune Regulator 1, ATPase, H+ Transporting, Lysosomal V0 Subunit A3 (TCIRG1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of T-Cell, Immune Regulator 1 (TCIRG1). No significant cross-reactivity or interference between T-Cell, Immune Regulator 1 (TCIRG1) and analogues was observed.

Sensitivity: 6.0 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to T-Cell, Immune Regulator 1 (TCIRG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain T-Cell, Immune Regulator 1 (TCIRG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of T-Cell, Immune Regulator 1 (TCIRG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level T-Cell, Immune Regulator 1 (TCIRG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for TCIRG1

Target: TCIRG1 (T-Cell, Immune Regulator 1, ATPase, H+ Transporting, Lysosomal V0 Subunit A3 (TCIRG1))

Alternative Name: T-Cell, Immune Regulator 1 (TCIRG1 Products)

Synonyms: OC116 ELISA Kit, TIRC7 ELISA Kit, ATP6N1C ELISA Kit, ATP6a3 ELISA Kit, Atp6i ELISA Kit, OC-116 ELISA Kit, OPTB1 ELISA Kit, Stv1 ELISA Kit, Vph1 ELISA Kit, oc ELISA Kit, ATP6V0A3 ELISA Kit, OC-116kDa ELISA Kit, a3 ELISA Kit, T-cell immune regulator 1, ATPase H+ transporting V0 subunit A3 ELISA Kit, T cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 protein A3 ELISA Kit, T-cell immune regulator 1, ATPase H+ transporting V0 subunit a3 ELISA Kit, Tcirg1 ELISA Kit, TCIRG1 ELISA Kit

Background:

Gene Name: T-Cell, Immune Regulator 1

Gene Aliases: Stv1, Vph1, ATP6N1C, ATP6V0A3, Atp6i, OC-116 kDa, OC116, OPTB1, TIRC7, a3, V-Type Proton ATPase 116 kDa Subunit A Isoform 3, Osteoclastic proton pump 116 kDa subunit

Pathways: Transition Metal Ion Homeostasis, Proton Transport