TXN CLIA Kit

Catalog No. ABIN5659146

Product Details TXN CLIA Kit

Target: TXN (Thioredoxin (TXN))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thioredoxin (Trx). No significant cross-reactivity or interference between Thioredoxin (Trx) and analogues was observed.

Sensitivity: 2.53 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Thioredoxin (Trx). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Thioredoxin (Trx). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Thioredoxin (Trx) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thioredoxin (Trx) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thioredoxin (Trx) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: TXN (Thioredoxin (TXN))

Alternative Name: Thioredoxin (TXN Products)

Synonyms: TRDX CLIA Kit, TRX CLIA Kit, TRX1 CLIA Kit, ADF CLIA Kit, AW550880 CLIA Kit, Trx1 CLIA Kit, Txn CLIA Kit, trx CLIA Kit, TXN CLIA Kit, SCM1.18 CLIA Kit, trxA3 CLIA Kit, thioredoxin CLIA Kit, TR-1 CLIA Kit, Trx-1 CLIA Kit, thioredoxin CLIA Kit, thioredoxin 1 CLIA Kit, thioredoxin L homeolog CLIA Kit, similar to 44RRORF109c hypothetical protein; similar to bacterial thioredoxin (SwissProt P06544) CLIA Kit, thioredoxin pseudogene CLIA Kit, cytosolic thioredoxin Trx1 CLIA Kit, TXN CLIA Kit, Txn1 CLIA Kit, txn.L CLIA Kit, txn CLIA Kit, trx CLIA Kit, SCO0885 CLIA Kit, PHG25ORF099c CLIA Kit, LOC100349046 CLIA Kit, Txn CLIA Kit, trx1 CLIA Kit

Background:

Gene Name: Thioredoxin

Gene Aliases: TXN, ADF, SASP, TRDX, TRX1, ATL-derived factor, Surface-associated sulphydryl protein

Gene ID: 7295

UniProt: P10599

Pathways: Carbohydrate Homeostasis, Cell RedoxHomeostasis