SERPINA7 ELISA Kit

Catalog No. ABIN5659254

Product Details SERPINA7 ELISA Kit

Target: SERPINA7 (Serpin Family A Member 7 (SERPINA7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Thyroxine Binding Globulin (TBG). No significant cross-reactivity or interference between Thyroxine Binding Globulin (TBG) and analogues was observed.

Sensitivity: 0.145 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thyroxine Binding Globulin (TBG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thyroxine Binding Globulin (TBG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thyroxine Binding Globulin (TBG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thyroxine Binding Globulin (TBG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thyroxine Binding Globulin (TBG) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thyroxine Binding Globulin (TBG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for SERPINA7

Target: SERPINA7 (Serpin Family A Member 7 (SERPINA7))

Alternative Name: Thyroxine Binding Globulin (SERPINA7 Products)

Synonyms: C730040N12Rik ELISA Kit, Tbg ELISA Kit, TBG ELISA Kit, THYROXINE-BINDING GLOBULIN ELISA Kit, serine (or cysteine) peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7 ELISA Kit, serpin family A member 7 ELISA Kit, Serpina7 ELISA Kit, SERPINA7 ELISA Kit

Background:

Gene Name: Thyroxine Binding Globulin

Gene Aliases: SERPINA7, Serpin Peptidase Inhibitor Clade A Member 7, T4-binding globulin

Gene ID: 331535

UniProt: P61939

Pathways: Hormone Transport