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Transferrin ELISA Kit

TF Reactivity: Pig Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Catalog No. ABIN5659405
  • Target See all Transferrin (TF) ELISA Kits
    Transferrin (TF)
    Reactivity
    • 7
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Pig
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.
    Sensitivity
    0.105 ng/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transferrin (TRF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transferrin (TRF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all Transferrin (TF) ELISA Kits
    Transferrin (TF)
    Alternative Name
    Transferrin (TF Products)
    Synonyms
    ltf ELISA Kit, pro1557 ELISA Kit, pro2086 ELISA Kit, mgc107777 ELISA Kit, LOC692564 ELISA Kit, TF ELISA Kit, LOC100144362 ELISA Kit, tf ELISA Kit, LTF ELISA Kit, TFEW ELISA Kit, conalbumin ELISA Kit, tf-b ELISA Kit, MGC64306 ELISA Kit, PRO1557 ELISA Kit, PRO2086 ELISA Kit, TFQTL1 ELISA Kit, AI266983 ELISA Kit, Cd176 ELISA Kit, HP ELISA Kit, Tf ELISA Kit, Tfn ELISA Kit, hpx ELISA Kit, Trf ELISA Kit, cb285 ELISA Kit, gavi ELISA Kit, id:ibd3238 ELISA Kit, id:ibd3525 ELISA Kit, sb:cb285 ELISA Kit, wu:fb57g06 ELISA Kit, wu:fb62h02 ELISA Kit, wu:fb63h10 ELISA Kit, wu:fb64h10 ELISA Kit, zgc:112154 ELISA Kit, 143958_at ELISA Kit, CG6186 ELISA Kit, Dmel\\CG6186 ELISA Kit, TSF1 ELISA Kit, anon-EST:Posey265 ELISA Kit, tsf1 ELISA Kit, Pro-TRH ELISA Kit, IL-5 ELISA Kit, STF I ELISA Kit, TRF1 ELISA Kit, sTF1 ELISA Kit, sTf ELISA Kit, tf1 ELISA Kit, transferrin ELISA Kit, serotransferrin ELISA Kit, transferrin (ovotransferrin) ELISA Kit, transferrin L homeolog ELISA Kit, melanotransferrin ELISA Kit, transferrin-a ELISA Kit, Transferrin 1 ELISA Kit, thyrotropin releasing hormone ELISA Kit, interleukin 5 ELISA Kit, TF ELISA Kit, LOC477072 ELISA Kit, tf ELISA Kit, Tf ELISA Kit, LOC100144362 ELISA Kit, tf.L ELISA Kit, LOC5575625 ELISA Kit, Trf ELISA Kit, tfa ELISA Kit, Tsf1 ELISA Kit, TRH ELISA Kit, IL5 ELISA Kit, LOC101085148 ELISA Kit, LOC100726872 ELISA Kit, trf ELISA Kit
    Background

    Gene Name: Transferrin

    Gene Aliases: TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin

    Pathways
    Transition Metal Ion Homeostasis
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