Transferrin CLIA Kit

Catalog No. ABIN5659406

Product Details Transferrin CLIA Kit

Target: Transferrin (TF)

Reactivity: Rat

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 40000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transferrin (TRF). No significant cross-reactivity or interference between Transferrin (TRF) and analogues was observed.

Sensitivity: 20.3 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Transferrin (TRF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transferrin (TRF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transferrin (TRF) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transferrin (TRF) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transferrin (TRF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: Transferrin (TF)

Alternative Name: Transferrin (TF Products)

Synonyms: ltf CLIA Kit, pro1557 CLIA Kit, pro2086 CLIA Kit, mgc107777 CLIA Kit, LOC692564 CLIA Kit, TF CLIA Kit, LOC100144362 CLIA Kit, tf CLIA Kit, LTF CLIA Kit, TFEW CLIA Kit, conalbumin CLIA Kit, tf-b CLIA Kit, MGC64306 CLIA Kit, PRO1557 CLIA Kit, PRO2086 CLIA Kit, TFQTL1 CLIA Kit, AI266983 CLIA Kit, Cd176 CLIA Kit, HP CLIA Kit, Tf CLIA Kit, Tfn CLIA Kit, hpx CLIA Kit, Trf CLIA Kit, cb285 CLIA Kit, gavi CLIA Kit, id:ibd3238 CLIA Kit, id:ibd3525 CLIA Kit, sb:cb285 CLIA Kit, wu:fb57g06 CLIA Kit, wu:fb62h02 CLIA Kit, wu:fb63h10 CLIA Kit, wu:fb64h10 CLIA Kit, zgc:112154 CLIA Kit, 143958_at CLIA Kit, CG6186 CLIA Kit, Dmel\\CG6186 CLIA Kit, TSF1 CLIA Kit, anon-EST:Posey265 CLIA Kit, tsf1 CLIA Kit, Pro-TRH CLIA Kit, IL-5 CLIA Kit, STF I CLIA Kit, TRF1 CLIA Kit, sTF1 CLIA Kit, sTf CLIA Kit, tf1 CLIA Kit, transferrin CLIA Kit, serotransferrin CLIA Kit, transferrin (ovotransferrin) CLIA Kit, transferrin L homeolog CLIA Kit, melanotransferrin CLIA Kit, transferrin-a CLIA Kit, Transferrin 1 CLIA Kit, thyrotropin releasing hormone CLIA Kit, interleukin 5 CLIA Kit, TF CLIA Kit, LOC477072 CLIA Kit, tf CLIA Kit, Tf CLIA Kit, LOC100144362 CLIA Kit, tf.L CLIA Kit, LOC5575625 CLIA Kit, Trf CLIA Kit, tfa CLIA Kit, Tsf1 CLIA Kit, TRH CLIA Kit, IL5 CLIA Kit, LOC101085148 CLIA Kit, LOC100726872 CLIA Kit, trf CLIA Kit

Background:

Gene Name: Transferrin

Gene Aliases: TF, Siderophilin, Serotransferrin, Beta-1 metal-binding globulin

UniProt: P12346

Pathways: Transition Metal Ion Homeostasis