TGFB1 CLIA Kit

Catalog No. ABIN5659432

Product Details TGFB1 CLIA Kit

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivity: Pig

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Transforming Growth Factor Beta 1 (TGFb1). No significant cross-reactivity or interference between Transforming Growth Factor Beta 1 (TGFb1) and analogues was observed.

Sensitivity: 5.7 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Transforming Growth Factor Beta 1 (TGFb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Transforming Growth Factor Beta 1 (TGFb1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transforming Growth Factor Beta 1 (TGFb1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Alternative Name: Transforming Growth Factor Beta 1 (TGFB1 Products)

Synonyms: CED CLIA Kit, DPD1 CLIA Kit, LAP CLIA Kit, TGFB CLIA Kit, TGFbeta CLIA Kit, TGF-beta CLIA Kit, TGF-BETA-1 CLIA Kit, TGF-beta5 CLIA Kit, ced CLIA Kit, dpd1 CLIA Kit, lap CLIA Kit, tgf-beta CLIA Kit, tgfb CLIA Kit, tgfb5 CLIA Kit, tgfbeta CLIA Kit, TGF-beta1 CLIA Kit, TGFbeta1 CLIA Kit, Tgfb CLIA Kit, Tgfb-1 CLIA Kit, ai39657 CLIA Kit, tgfb1 CLIA Kit, wu:fb13a07 CLIA Kit, xx:ai39657 CLIA Kit, TGFB1 CLIA Kit, csd CLIA Kit, cdb1 CLIA Kit, cdg2 CLIA Kit, csd1 CLIA Kit, csd2 CLIA Kit, csd3 CLIA Kit, ebmd CLIA Kit, lcd1 CLIA Kit, bigh3 CLIA Kit, cdgg1 CLIA Kit, betaig-h3 CLIA Kit, TGFB4 CLIA Kit, transforming growth factor beta 1 CLIA Kit, transforming growth factor beta-1 CLIA Kit, transforming growth factor beta 1 L homeolog CLIA Kit, transforming growth factor, beta 1 CLIA Kit, transforming growth factor, beta 1a CLIA Kit, transforming growth factor beta induced L homeolog CLIA Kit, TGFB1 CLIA Kit, Tgfb1 CLIA Kit, tgfb1.L CLIA Kit, tgfb1a CLIA Kit, tgfbi.L CLIA Kit

Background:

Gene Name: Transforming Growth Factor Beta 1

Gene Aliases: TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide

Gene ID: 397078

UniProt: P07200

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints