Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

14-3-3 zeta ELISA Kit

YWHAZ Reactivity: Human, Rat, Mouse Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5659710
  • Target See all 14-3-3 zeta (YWHAZ) ELISA Kits
    14-3-3 zeta (YWHAZ)
    Reactivity
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). No significant cross-reactivity or interference between Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) and analogues was observed.
    Sensitivity
    0.116 ng/mL
    Featured
    Discover our best selling YWHAZ ELISA Kit
    Top Product
    Discover our top product YWHAZ ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAz) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all 14-3-3 zeta (YWHAZ) ELISA Kits
    14-3-3 zeta (YWHAZ)
    Alternative Name
    Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta (YWHAZ Products)
    Synonyms
    14-3-3-zeta ELISA Kit, KCIP-1 ELISA Kit, YWHAD ELISA Kit, 14-3-3zeta ELISA Kit, Ywhaz ELISA Kit, ACYPI003154 ELISA Kit, 14-3-3z ELISA Kit, kcip-1 ELISA Kit, ywhaq ELISA Kit, 1433z ELISA Kit, ywhaz ELISA Kit, ywhazb ELISA Kit, 1110013I11Rik ELISA Kit, AI596267 ELISA Kit, AL022924 ELISA Kit, AU020854 ELISA Kit, ywhaza ELISA Kit, fb14h09 ELISA Kit, wu:fb05g08 ELISA Kit, wu:fb14h09 ELISA Kit, ywhai ELISA Kit, zgc:55807 ELISA Kit, 14-3-3 ELISA Kit, 14-3-3 zeta ELISA Kit, 14-3-3ZETA ELISA Kit, 14-3-3leo ELISA Kit, 2G1 ELISA Kit, 4-3-3 zeta ELISA Kit, 5.11 ELISA Kit, 549 ELISA Kit, BEST:GH05075 ELISA Kit, CG17870 ELISA Kit, D14-3-3 ELISA Kit, D14-3-3zeta ELISA Kit, Dmel\\CG17870 ELISA Kit, K ELISA Kit, LEO ELISA Kit, Leo ELISA Kit, PAR-5 ELISA Kit, PAR5 ELISA Kit, Par-5 ELISA Kit, d14-3-3zeta ELISA Kit, l(2)07103 ELISA Kit, l(2)46CFe ELISA Kit, l(2)46Ee ELISA Kit, leo ELISA Kit, par-5 ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta ELISA Kit, 14-3-3 protein zeta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta L homeolog ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta ELISA Kit, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta S homeolog ELISA Kit, 14-3-3 protein zeta/delta pseudogene ELISA Kit, CG17870 gene product from transcript CG17870-RE ELISA Kit, YWHAZ ELISA Kit, 14-3-3zeta ELISA Kit, ywhaz ELISA Kit, 1433z ELISA Kit, ywhaz.L ELISA Kit, Ywhaz ELISA Kit, ywhaz.S ELISA Kit, LOC100855903 ELISA Kit
    Background

    Gene Name: Tyrosine 3/Tryptophan 5 Monooxygenase Activation Protein Zeta

    Gene Aliases: KCIP-1, 14-3-3-zeta, 14-3-3 protein zeta/delta, Protein kinase C inhibitor protein 1

    Pathways
    Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
You are here:
Support