UBA52 ELISA Kit

Catalog No. ABIN5659724

Product Details UBA52 ELISA Kit

Target: UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). No significant cross-reactivity or interference between Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) and analogues was observed.

Sensitivity: 0.116 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for UBA52

Target: UBA52 (Ubiquitin A-52 Residue Ribosomal Protein Fusion Product 1 (UBA52))

Alternative Name: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1 (UBA52 Products)

Synonyms: CEP52 ELISA Kit, HUBCEP52 ELISA Kit, L40 ELISA Kit, RPL40 ELISA Kit, D8Ertd21e ELISA Kit, Gm1863 ELISA Kit, Rps27a ELISA Kit, Ubb ELISA Kit, Ubc ELISA Kit, UBCEP2 ELISA Kit, UB-RPL40 ELISA Kit, MGC80109 ELISA Kit, cep52 ELISA Kit, hubcep52 ELISA Kit, rpl40 ELISA Kit, RPS27A ELISA Kit, UBB ELISA Kit, UBC ELISA Kit, wu:fa91f08 ELISA Kit, zgc:123344 ELISA Kit, UBA52 ELISA Kit, ubiquitin A-52 residue ribosomal protein fusion product 1 ELISA Kit, ubiquitin A-52 residue ribosomal protein fusion product 1 L homeolog ELISA Kit, UBA52 ELISA Kit, Uba52 ELISA Kit, uba52.L ELISA Kit, uba52 ELISA Kit

Background:

Gene Name: Ubiquitin A 52 Residue Ribosomal Protein Fusion Product 1

Gene Aliases: CEP52, HUBCEP52, RPL40, 60S Ribosomal Protein L40, Ubiquitin Carboxyl Extension Protein 52

Gene ID: 7311

UniProt: P62987

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation