VCP ELISA Kit

Catalog No. ABIN5659824

Product Details VCP ELISA Kit

Target: VCP (Valosin Containing Protein (VCP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Valosin Containing Protein (VCP). No significant cross-reactivity or interference between Valosin Containing Protein (VCP) and analogues was observed.

Sensitivity: 0.112 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Valosin Containing Protein (VCP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Valosin Containing Protein (VCP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Valosin Containing Protein (VCP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Valosin Containing Protein (VCP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Valosin Containing Protein (VCP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: Valosin Containing Protein (VCP Products)

Synonyms: ALS14 ELISA Kit, IBMPFD ELISA Kit, TERA ELISA Kit, p97 ELISA Kit, CDC48 ELISA Kit, wu:fd16d05 ELISA Kit, wu:fj63d11 ELISA Kit, p93 ELISA Kit, 3110001E05 ELISA Kit, p97/VCP ELISA Kit, 22.26 ELISA Kit, 7.12 ELISA Kit, Apple ELISA Kit, BcDNA.GM02885 ELISA Kit, BcDNA:GM02885 ELISA Kit, CG2331 ELISA Kit, DmTER94 ELISA Kit, Dmel\CG2331 ELISA Kit, I ELISA Kit, Ter94 ELISA Kit, Tera ELISA Kit, VCP ELISA Kit, VCP ATPase ELISA Kit, VCP/p97 ELISA Kit, Vcp ELISA Kit, anon-WO2004063362.65 ELISA Kit, anon-WO2004063362.67 ELISA Kit, cdc48 ELISA Kit, dTERA ELISA Kit, dVCP ELISA Kit, dmTERA ELISA Kit, dvcp ELISA Kit, l(2)03775 ELISA Kit, l(2)46CFf ELISA Kit, l(2)46CFs ELISA Kit, l(2)46Ch ELISA Kit, pTER94 ELISA Kit, ter94 ELISA Kit, vcp ELISA Kit, FANCG ELISA Kit, valosin containing protein ELISA Kit, valosin containing protein S homeolog ELISA Kit, valosin-containing protein ELISA Kit, CG2331 gene product from transcript CG2331-RA ELISA Kit, zgc:136908 ELISA Kit, transitional endoplasmic reticulum ATPase ELISA Kit, VCP ELISA Kit, vcp.S ELISA Kit, vcp ELISA Kit, Vcp ELISA Kit, Bm1_56585 ELISA Kit, LOC100136246 ELISA Kit, TER94 ELISA Kit, zgc:136908 ELISA Kit, LOC101109680 ELISA Kit

Background:

Gene Name: Valosin Containing Protein

Gene Aliases: IBMPFD, p97, TERA, Transitional Endoplasmic Reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway