Vitamin D Receptor ELISA Kit

Catalog No. ABIN5659959

Product Details Vitamin D Receptor ELISA Kit

Target: Vitamin D Receptor (VDR)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.625 ng/mL - 40 ng/mL

Minimum Detection Limit: 0.625 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Vitamin D Receptor (VDR). No significant cross-reactivity or interference between Vitamin D Receptor (VDR) and analogues was observed.

Sensitivity: 0.248 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Vitamin D Receptor (VDR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Vitamin D Receptor (VDR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Vitamin D Receptor (VDR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Vitamin D Receptor (VDR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin D Receptor (VDR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for Vitamin D Receptor

Target: Vitamin D Receptor (VDR)

Alternative Name: Vitamin D Receptor (VDR Products)

Synonyms: vdrbeta ELISA Kit, vdr0 ELISA Kit, LOC100136219 ELISA Kit, NR1I1-B ELISA Kit, gb:dq017633 ELISA Kit, vdr-b ELISA Kit, Ci-VDR-b ELISA Kit, VDR ELISA Kit, LOC100221284 ELISA Kit, vdr-A ELISA Kit, xVDR ELISA Kit, Nr1i1 ELISA Kit, vdr ELISA Kit, NR1I1 ELISA Kit, PPP1R163 ELISA Kit, vitamin D (1,25- dihydroxyvitamin D3) receptor ELISA Kit, vitamin D receptor ELISA Kit, vitamin D3 receptor A ELISA Kit, vitamin D receptor b ELISA Kit, nuclear receptor VDR-b ELISA Kit, vitamin D (1,25- dihydroxyvitamin D3) receptor L homeolog ELISA Kit, vitamin D (1,25-dihydroxyvitamin D3) receptor ELISA Kit, vitamin D receptor a ELISA Kit, vdr ELISA Kit, vdr0 ELISA Kit, VDR ELISA Kit, LOC100136219 ELISA Kit, vdrb ELISA Kit, vdr-b ELISA Kit, vdr.L ELISA Kit, Vdr ELISA Kit, vdra ELISA Kit

Target Type: Chemical

Background:

Gene Name: Vitamin D Receptor

Gene Aliases: NR1I1, Nuclear Receptor Subfamily 1 Group I Member 1, Calcitriol Receptor, 1,25-dihydroxyvitamin D3 receptor

Gene ID: 7421

UniProt: P11473

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway