VDAC1 ELISA Kit

Catalog No. ABIN5659974

Product Details VDAC1 ELISA Kit

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Voltage Dependent Anion Channel Protein 1 (VDAC1). No significant cross-reactivity or interference between Voltage Dependent Anion Channel Protein 1 (VDAC1) and analogues was observed.

Sensitivity: 0.131 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Voltage Dependent Anion Channel Protein 1 (VDAC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Voltage Dependent Anion Channel Protein 1 (VDAC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Voltage Dependent Anion Channel Protein 1 (VDAC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Voltage Dependent Anion Channel Protein 1 (VDAC1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for VDAC1

Target: VDAC1 (Voltage-Dependent Anion Channel 1 (VDAC1))

Alternative Name: Voltage Dependent Anion Channel Protein 1 (VDAC1 Products)

Synonyms: vdac1 ELISA Kit, VDAC1 ELISA Kit, 5076 ELISA Kit, ARABIDOPSIS THALIANA VOLTAGE DEPENDENT ANION CHANNEL 1 ELISA Kit, ATVDAC1 ELISA Kit, T22N4.9 ELISA Kit, T22N4_9 ELISA Kit, voltage dependent anion channel 1 ELISA Kit, PORIN ELISA Kit, VDAC-1 ELISA Kit, AL033343 ELISA Kit, Vdac5 ELISA Kit, fa13f11 ELISA Kit, wu:fa13f11 ELISA Kit, zgc:85830 ELISA Kit, voltage-dependent anion channel 1 S homeolog ELISA Kit, voltage-dependent anion channel 1 ELISA Kit, voltage dependent anion channel 1 ELISA Kit, vdac1.S ELISA Kit, vdac1 ELISA Kit, VDAC1 ELISA Kit, Vdac1 ELISA Kit

Background:

Gene Name: Voltage Dependent Anion Channel Protein 1

Gene Aliases: PORIN-31-HL, Porin 31HM, Plasmalemmal porin, Outer mitochondrial membrane protein porin 1, Voltage-dependent anion-selective channel protein 1

Gene ID: 7416

UniProt: P21796