WNT5B ELISA Kit

Catalog No. ABIN5660022

Product Details WNT5B ELISA Kit

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) and analogues was observed.

Sensitivity: 0.55 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 5B (WNT5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 5B (WNT5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 5B (WNT5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for WNT5B

Target: WNT5B (Wingless-Type MMTV Integration Site Family, Member 5B (WNT5B))

Alternative Name: Wingless Type MMTV Integration Site Family, Member 5B (WNT5B Products)

Synonyms: wnt-5b ELISA Kit, xwnt5b ELISA Kit, WNT5B ELISA Kit, AW545702 ELISA Kit, Wnt-5b ELISA Kit, xwnt-5c ELISA Kit, CHUNP6928 ELISA Kit, id:ibd5111 ELISA Kit, ppt ELISA Kit, wnt-5 ELISA Kit, wnt5 ELISA Kit, wnt[b] ELISA Kit, wu:fk85g06 ELISA Kit, Wnt family member 5B ELISA Kit, wingless-type MMTV integration site family, member 5B ELISA Kit, Wnt family member 5B S homeolog ELISA Kit, wingless-type MMTV integration site family, member 5b ELISA Kit, wnt5b ELISA Kit, WNT5B ELISA Kit, Wnt5b ELISA Kit, wnt5b.S ELISA Kit

Background:

Gene Name: Wingless Type MMTV Integration Site Family, Member 5B

Pathways: WNT Signaling, Embryonic Body Morphogenesis, Positive Regulation of fat Cell Differentiation