WIF1 CLIA Kit

Catalog No. ABIN5660030

Product Details WIF1 CLIA Kit

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of WNT Inhibitory Factor 1 (WIF1). No significant cross-reactivity or interference between WNT Inhibitory Factor 1 (WIF1) and analogues was observed.

Sensitivity: 0.55 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the WNT Inhibitory Factor 1 (WIF1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT Inhibitory Factor 1 (WIF1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Alternative Name: WNT Inhibitory Factor 1 (WIF1 Products)

Synonyms: shf CLIA Kit, GB10372 CLIA Kit, wif-1 CLIA Kit, WIF1 CLIA Kit, WIF-1 CLIA Kit, AW107799 CLIA Kit, zgc:110507 CLIA Kit, protein shifted CLIA Kit, wnt inhibitory factor 1 CLIA Kit, WNT inhibitory factor 1 CLIA Kit, Wnt inhibitory factor 1 CLIA Kit, WNT inhibitory factor 1 S homeolog CLIA Kit, LOC552805 CLIA Kit, CpipJ_CPIJ003761 CLIA Kit, wif1 CLIA Kit, WIF1 CLIA Kit, Wif1 CLIA Kit, LOC100511326 CLIA Kit, wif1.S CLIA Kit

Background:

Gene Name: WNT Inhibitory Factor 1

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation