XBP1 CLIA Kit

Catalog No. ABIN5660046

Product Details XBP1 CLIA Kit

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivity: Human

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 156.25 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of X-Box Binding Protein 1 (XBP1). No significant cross-reactivity or interference between X-Box Binding Protein 1 (XBP1) and analogues was observed.

Sensitivity: 5.19 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Box Binding Protein 1 (XBP1) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level X-Box Binding Protein 1 (XBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: XBP1 (X-Box Binding Protein 1 (XBP1))

Alternative Name: X-Box Binding Protein 1 (XBP1 Products)

Synonyms: treb5 CLIA Kit, xXBP1 CLIA Kit, xbp2 CLIA Kit, XBP1P1 CLIA Kit, XBPP1 CLIA Kit, xbp1 CLIA Kit, TREB5 CLIA Kit, XBP-1 CLIA Kit, XBP2 CLIA Kit, D11Ertd39e CLIA Kit, TREB-5 CLIA Kit, HTF CLIA Kit, cb918 CLIA Kit, id:ibd1195 CLIA Kit, trebf CLIA Kit, wu:fb02h05 CLIA Kit, wu:fb63f10 CLIA Kit, xbp1a CLIA Kit, xbp1b CLIA Kit, X-box binding protein 1 CLIA Kit, X-box binding protein 1 L homeolog CLIA Kit, xbp1 CLIA Kit, XBP1 CLIA Kit, Xbp1 CLIA Kit, xbp1.L CLIA Kit

Background:

Gene Name: X-Box Binding Protein 1

Gene Aliases: TREB5, XBP2, Tax-responsive element-binding protein 5

Pathways: ER-Nucleus Signaling, Unfolded Protein Response