COL3A1 ELISA Kit

Catalog No. ABIN5674676

Product Details COL3A1 ELISA Kit

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 14.5 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for COL3A1

Target: COL3A1 (Collagen, Type III, alpha 1 (COL3A1))

Alternative Name: Collagen Type III Alpha 1 (COL3a1) (COL3A1 Products)

Synonyms: EDS4A ELISA Kit, AW550625 ELISA Kit, Col3a-1 ELISA Kit, MMS10-W ELISA Kit, Ms10w ELISA Kit, mKIAA4231 ELISA Kit, col3a1 ELISA Kit, collagen type III alpha 1 chain ELISA Kit, collagen, type III, alpha 1 ELISA Kit, collagen, type III, alpha 1 (Ehlers-Danlos syndrome type IV, autosomal dominant) S homeolog ELISA Kit, COL3A1 ELISA Kit, Col3a1 ELISA Kit, col3a1 ELISA Kit, col3a1.S ELISA Kit

Background: Alternative name: COL3-A1, COL3A1, EDS4A, Collagen Type III Alpha 1, Ehlers-Danlos Syndrome Type IV Autosomal Dominant, Collagen Alpha-1(III)chain

Gene ID: 12825

UniProt: P08121

Pathways: Autophagy, Growth Factor Binding