TRPM7 ELISA Kit

Catalog No. ABIN5674682

Product Details TRPM7 ELISA Kit

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 34 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Alternative Name: Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) (TRPM7 Products)

Synonyms: cb495 ELISA Kit, TRPM7 ELISA Kit, chak ELISA Kit, chak1 ELISA Kit, ltrpc7 ELISA Kit, trp-plik ELISA Kit, ALSPDC ELISA Kit, CHAK ELISA Kit, CHAK1 ELISA Kit, LTRPC7 ELISA Kit, TRP-PLIK ELISA Kit, 2310022G15Rik ELISA Kit, 4833414K03Rik ELISA Kit, 5033407O22Rik ELISA Kit, LTrpC-7 ELISA Kit, Ltpr7 ELISA Kit, Ltrpc7 ELISA Kit, TRPPLIK ELISA Kit, Chak ELISA Kit, Trp-plik ELISA Kit, transient receptor potential cation channel subfamily M member 7 ELISA Kit, transient receptor potential cation channel, subfamily M, member 7 ELISA Kit, TRPM7 ELISA Kit, trpm7 ELISA Kit, Trpm7 ELISA Kit

Background: Alternative name: CHAK, CHAK1, LTRPC7, TRP-PLIK, Channel-kinase 1, Long transient receptor potential channel 7

Gene ID: 58800

UniProt: Q923J1