ATP6AP2 ELISA Kit

Catalog No. ABIN5674751

Product Details ATP6AP2 ELISA Kit

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0112 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Abstract: ATP6AP2 Products

Synonyms: APT6M8-9 ELISA Kit, ATP6IP2 ELISA Kit, ATP6M8-9 ELISA Kit, ELDF10 ELISA Kit, M8-9 ELISA Kit, MRXE ELISA Kit, MSTP009 ELISA Kit, XMRE ELISA Kit, (P)RR ELISA Kit, 5730403E06Rik ELISA Kit, Atp6ip2 ELISA Kit, tj6 ELISA Kit, wss ELISA Kit, arcl ELISA Kit, j6b7 ELISA Kit, stv1 ELISA Kit, tj6m ELISA Kit, tj6s ELISA Kit, vph1 ELISA Kit, atp6a2 ELISA Kit, atp6n1d ELISA Kit, ATP6AP2 ELISA Kit, DKFZp459G0918 ELISA Kit, atp6ap2 ELISA Kit, wu:fj36c03 ELISA Kit, wu:fq15e04 ELISA Kit, zgc:73194 ELISA Kit, ATPase H+ transporting accessory protein 2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 ELISA Kit, ATPase H+ transporting V0 subunit d2 ELISA Kit, ATPase H+ transporting V0 subunit a2 ELISA Kit, ATPase, H+ transporting, lysosomal accessory protein 2 S homeolog ELISA Kit, ATP6AP2 ELISA Kit, Atp6ap2 ELISA Kit, ATP6V0D2 ELISA Kit, atp6v0a2 ELISA Kit, atp6ap2.S ELISA Kit, atp6ap2 ELISA Kit

Background: Alternative name: ATP6IP2, CAPER, ELDF10, XMRE, Renin/prorenin receptor, Embryonic liver differentiation factor 10, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gene ID: 10159

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones