ENPP2 ELISA Kit

Catalog No. ABIN5674754

Product Details ENPP2 ELISA Kit

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0065 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ENPP2

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Abstract: ENPP2 Products

Synonyms: ATX ELISA Kit, ATX-X ELISA Kit, AUTOTAXIN ELISA Kit, LysoPLD ELISA Kit, NPP2 ELISA Kit, PD-IALPHA ELISA Kit, PDNP2 ELISA Kit, Npps2 ELISA Kit, PD-Ialpha ELISA Kit, Pdnp2 ELISA Kit, MGC132047 ELISA Kit, atx ELISA Kit, zgc:63550 ELISA Kit, autotaxin ELISA Kit, enpp2 ELISA Kit, enpp2a ELISA Kit, enpp2b ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 L homeolog ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 S homeolog ELISA Kit, ENPP2 ELISA Kit, Enpp2 ELISA Kit, enpp2 ELISA Kit, enpp2.L ELISA Kit, enpp2.S ELISA Kit

Background: Alternative name: ATX, ATX-X, LysoPLD, NPP2, PD-IALPHA, PDNP2, Autotaxin, Extracellular lysophospholipase D

Gene ID: 18606

UniProt: Q9R1E6