ATP4b ELISA Kit

Catalog No. ABIN5674849

Product Details ATP4b ELISA Kit

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0136 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ATP4b

Target: ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))

Alternative Name: ATPase, H+/K+ Exchanging Beta Polypeptide (ATP4b) (ATP4b Products)

Synonyms: AV080843 ELISA Kit, S76401S1 ELISA Kit, ATP6B ELISA Kit, ATPase, H+/K+ exchanging, beta polypeptide ELISA Kit, ATPase H+/K+ transporting beta subunit ELISA Kit, ATPase, H+/K+ exchanging, beta polypeptide S homeolog ELISA Kit, ATPase H+/K+ transporting alpha subunit ELISA Kit, Atp4b ELISA Kit, atp4b.S ELISA Kit, ATP4B ELISA Kit, ATP4A ELISA Kit

Background: Alternative name: Proton pump beta chain, Gastric H(+)/K(+) ATPase subunit beta

Gene ID: 24217

UniProt: P18598

Pathways: Proton Transport