ARPC4 ELISA Kit
ARPC4
Reactivity: Human
Colorimetric
Sandwich ELISA
0.312 ng/mL - 20 ng/mL
Tissue Homogenate
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- Target See all ARPC4 ELISA Kits
- ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.312 ng/mL - 20 ng/mL
- Minimum Detection Limit
- 0.312 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Sensitivity
- 0115 ng/mL
- Components
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - Material not included
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product ARPC4 ELISA Kit
-
Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4) ELISA Kit
ARPC4 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Assay Time
- 1 - 4.5 h
- Plate
- Pre-coated
- Protocol
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - Restrictions
- For Research Use only
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- Storage
- 4 °C
- Expiry Date
- 12 months
-
- Target See all ARPC4 ELISA Kits
- ARPC4 (Actin Related Protein 2/3 Complex, Subunit 4, 20kDa (ARPC4))
- Alternative Name
- Actin Related Protein 2/3 Complex Subunit 4 (ARPC4) (ARPC4 Products)
- Synonyms
- ARC20 ELISA Kit, P20-ARC ELISA Kit, 20kDa ELISA Kit, 5330419I20Rik ELISA Kit, AI327076 ELISA Kit, p20-Arc ELISA Kit, arc20 ELISA Kit, p20-arc ELISA Kit, ARPC4 ELISA Kit, fa07b12 ELISA Kit, wu:fa07b12 ELISA Kit, zgc:77932 ELISA Kit, actin related protein 2/3 complex subunit 4 ELISA Kit, Actin-related protein 2/3 complex subunit 4 ELISA Kit, actin-related protein 2/3 complex subunit 4 ELISA Kit, actin related protein 2/3 complex, subunit 4 ELISA Kit, actin related protein 2/3 complex subunit 4 S homeolog ELISA Kit, actin related protein 2/3 complex, subunit 4, 20kDa ELISA Kit, ARPC4 ELISA Kit, PTRG_00644 ELISA Kit, CMU_000110 ELISA Kit, Arpc4 ELISA Kit, arpc4 ELISA Kit, arpc4.S ELISA Kit
- Background
- Alternative name: ARC20, p20-Arc, Arp2/3 Protein Complex Subunit p20, Arp2/3 complex 20 kDa subunit
- Gene ID
- 100526693
- UniProt
- P59998
- Pathways
- RTK Signaling, Regulation of Actin Filament Polymerization
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