PPP3R1 ELISA Kit

Catalog No. ABIN5674873

Product Details PPP3R1 ELISA Kit

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Binding Specificity: Regulatory Subunit 1

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25 pg/mL - 2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 15.5 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for PPP3R1

Target: PPP3R1 (Protein Phosphatase 3, Regulatory Subunit B, alpha (PPP3R1))

Alternative Name: Protein Phosphatase 3, (PPP3R1) (PPP3R1 Products)

Synonyms: si:ch211-274c22.8 ELISA Kit, canb ELISA Kit, CALNB1 ELISA Kit, CNB ELISA Kit, CNB1 ELISA Kit, CaNB1 ELISA Kit, Cnb1 ELISA Kit, MCIP1 ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha a ELISA Kit, protein phosphatase 3 regulatory subunit B, alpha S homeolog ELISA Kit, protein phosphatase 3 regulatory subunit B, alpha ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha ELISA Kit, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I) ELISA Kit, ppp3r1a ELISA Kit, ppp3r1.S ELISA Kit, PPP3R1 ELISA Kit, Ppp3r1 ELISA Kit

Background: Alternative name: CALNB1, CNB, CNB1, Calcineurin B Type I, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

Gene ID: 5534

UniProt: P63098

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling