ELAVL1 ELISA Kit

Catalog No. ABIN5674885

Product Details ELAVL1 ELISA Kit

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0118 ng/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ELAVL1

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Alternative Name: ELAV Like Protein 1 (ELAVL1) (ELAVL1 Products)

Synonyms: ELAV1 ELISA Kit, HUR ELISA Kit, Hua ELISA Kit, MelG ELISA Kit, HuR ELISA Kit, 2410055N02Rik ELISA Kit, W91709 ELISA Kit, elra ELISA Kit, fa25f08 ELISA Kit, fb59a02 ELISA Kit, fc60g02 ELISA Kit, hua ELISA Kit, wu:fa25f08 ELISA Kit, wu:fb59a02 ELISA Kit, wu:fc60g02 ELISA Kit, zHuA ELISA Kit, Elavl1 ELISA Kit, Xel-1 ELISA Kit, elav1 ELISA Kit, elrA ELISA Kit, hur ELISA Kit, melg ELISA Kit, p36 ELISA Kit, ElrA ELISA Kit, elavl1 ELISA Kit, elavl1-a ELISA Kit, elavl1-b ELISA Kit, ELAV like RNA binding protein 1 ELISA Kit, ELAV-like protein 1 ELISA Kit, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) ELISA Kit, ELAV like RNA binding protein 1a ELISA Kit, ELAV like RNA binding protein 1 L homeolog ELISA Kit, ELAV like RNA binding protein 1 S homeolog ELISA Kit, ELAVL1 ELISA Kit, Elavl1 ELISA Kit, elav1 ELISA Kit, elavl1a ELISA Kit, elavl1.L ELISA Kit, elavl1.S ELISA Kit

Background: Alternative name: ELAV1, HUR, Hua, MelG, Hu Antigen R, Embryonic Lethal,Abnormal Vision Like 1

Gene ID: 1994

UniProt: Q15717

Pathways: AMPK Signaling