Target See all HADHB ELISA Kits: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))
Reactivity 2 2 2 1: Mouse
Detection Method: Colorimetric
Method Type: Sandwich ELISA
Detection Range: 0.312 ng/mL - 20 ng/mL
Minimum Detection Limit: 0.312 ng/mL
Application: ELISA
Sample Type: Tissue Homogenate
Analytical Method: Quantitative
Sensitivity: 0115 ng/mL
Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual
Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
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HADHB Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate

HADHB Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate

HADHB Reactivity: Human Mitochondrial Colorimetric 0.156-10 ng/mL

HADHB Reactivity: Mouse Mitochondrial Colorimetric

HADHB Reactivity: Rat Mitochondrial Colorimetric

HADHB Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate

HADHB Reactivity: Cow Mitochondrial Colorimetric

Application Notes: Optimal working dilution should be determined by the investigator.
Assay Time: 1 - 4.5 h
Plate: Pre-coated
Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.
Restrictions: For Research Use only

Target See all HADHB ELISA Kits: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))
Alternative Name: Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) (HADHB Products)
Synonyms: ECHB ELISA Kit, MTPB ELISA Kit, TP-BETA ELISA Kit, thiolase ELISA Kit, fb14g10 ELISA Kit, wu:fb14g10 ELISA Kit, zgc:56274 ELISA Kit, 4930479F15Rik ELISA Kit, Mtpb ELISA Kit, Hadhb ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta S homeolog ELISA Kit, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit ELISA Kit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit ELISA Kit, HADHB ELISA Kit, hadhb.S ELISA Kit, Hadhb ELISA Kit, hadhb ELISA Kit
Background: Alternative name: MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase
Gene ID: 231086
UniProt: Q99JY0
Pathways: Monocarboxylic Acid Catabolic Process

HADHB ELISA Kit

Product Details HADHB ELISA Kit