SLC30A1 ELISA Kit

Catalog No. ABIN5674928

Product Details SLC30A1 ELISA Kit

Target: SLC30A1 (Solute Carrier Family 30 (Zinc Transporter), Member 1 (SLC30A1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.125 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.125 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 32 pg/mL

Components: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Material not included: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for SLC30A1

Target: SLC30A1 (Solute Carrier Family 30 (Zinc Transporter), Member 1 (SLC30A1))

Alternative Name: Solute Carrier Family 30, Member 1 (SLC30A1) (SLC30A1 Products)

Synonyms: ZNT1 ELISA Kit, ZRC1 ELISA Kit, AI839647 ELISA Kit, C130040I11Rik ELISA Kit, Znt1 ELISA Kit, ZnT1 ELISA Kit, slc30 ELISA Kit, MGC53047 ELISA Kit, XZnSLC30 ELISA Kit, slc30a1 ELISA Kit, zgc:77589 ELISA Kit, zrc1 ELISA Kit, ZnT-1 ELISA Kit, ZNT8 ELISA Kit, ZnT-8 ELISA Kit, zinc transporter 1 precursor ELISA Kit, RGD1305098 ELISA Kit, zgc:162909 ELISA Kit, znt1 ELISA Kit, solute carrier family 30 member 1 ELISA Kit, solute carrier family 30 (zinc transporter), member 1 ELISA Kit, solute carrier family 30 member 1 L homeolog ELISA Kit, solute carrier family 30 (zinc transporter), member 1a ELISA Kit, solute carrier family 30 member 8 ELISA Kit, zinc transporter 1 precursor ELISA Kit, solute carrier family 30, member 10 ELISA Kit, solute carrier family 30 (zinc transporter), member 1b ELISA Kit, SLC30A1 ELISA Kit, Slc30a1 ELISA Kit, slc30a1.L ELISA Kit, slc30a1a ELISA Kit, slc30a1 ELISA Kit, SLC30A8 ELISA Kit, ZIP1 ELISA Kit, Slc30a10 ELISA Kit, slc30a1b ELISA Kit

Background: Alternative name: ZNT1, ZRC1, Zinc Transporter 1

Gene ID: 58976

UniProt: Q62720

Pathways: Transition Metal Ion Homeostasis