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LKB1 ELISA Kit

STK11 Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5674931
  • Target See all LKB1 (STK11) ELISA Kits
    LKB1 (STK11) (serine/threonine Kinase 11 (STK11))
    Reactivity
    • 8
    • 3
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Sensitivity
    0057 ng/mL
    Components
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Material not included
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Assay Time
    1 - 4.5 h
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Expiry Date
    12 months
  • Target See all LKB1 (STK11) ELISA Kits
    LKB1 (STK11) (serine/threonine Kinase 11 (STK11))
    Abstract
    STK11 Products
    Synonyms
    pjs ELISA Kit, LKB1 ELISA Kit, XEEK1 ELISA Kit, Stk11 ELISA Kit, PJS ELISA Kit, hLKB1 ELISA Kit, AA408040 ELISA Kit, Lkb1 ELISA Kit, Par-4 ELISA Kit, R75140 ELISA Kit, mLKB1 ELISA Kit, wu:fj61a05 ELISA Kit, zgc:110180 ELISA Kit, xeek1 ELISA Kit, serine/threonine kinase 11 ELISA Kit, polarization-related protein LKB1 ELISA Kit, serine/threonine kinase 11 L homeolog ELISA Kit, STK11 ELISA Kit, stk11 ELISA Kit, LOC662493 ELISA Kit, Stk11 ELISA Kit, stk11.L ELISA Kit
    Background
    Alternative name: LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19
    UniProt
    D4AE59
    Pathways
    AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect
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