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CGRP ELISA Kit

CALCA Reactivity: Human, Rat, Mouse Colorimetric Competition ELISA 0.1 ng/mL - 1000 ng/mL Cell Culture Supernatant, Serum
Catalog No. ABIN5690744
  • Target See all CGRP (CALCA) ELISA Kits
    CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
    Reactivity
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Detection Range
    0.1 ng/mL - 1000 ng/mL
    Minimum Detection Limit
    0.1 ng/mL
    Application
    ELISA
    Purpose
    Human/Mouse/Rat CGRP-I EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
    Sample Type
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This EIA kit is designed to detect human, mouse, and rat Dynorphin
    Sensitivity
    0.2 ng/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Reagent Preparation

    Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction

    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Expiry Date
    6 months
  • Target See all CGRP (CALCA) ELISA Kits
    CGRP (CALCA) (Calcitonin-Related Polypeptide alpha (CALCA))
    Alternative Name
    CGRP-I (CALCA Products)
    Synonyms
    CALC1 ELISA Kit, CGRP ELISA Kit, CGRP-I ELISA Kit, CGRP1 ELISA Kit, CT ELISA Kit, KC ELISA Kit, CALC ELISA Kit, CALCA ELISA Kit, CALC3 ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, CA ELISA Kit, CGRP-1 ELISA Kit, Calc ELISA Kit, Calc1 ELISA Kit, Cgrp ELISA Kit, Ct ELISA Kit, Ctn ELISA Kit, calcitonin ELISA Kit, CAL6 ELISA Kit, Cal1 ELISA Kit, RATCAL6 ELISA Kit, CGRPI ELISA Kit, calc ELISA Kit, cgrp ELISA Kit, zgc:92886 ELISA Kit, calcitonin related polypeptide alpha ELISA Kit, calcitonin related polypeptide beta ELISA Kit, calcitonin-related polypeptide alpha ELISA Kit, calcitonin ELISA Kit, calcitonin/calcitonin-related polypeptide, alpha ELISA Kit, calcitonin-related polypeptide beta ELISA Kit, calcitonin gene-related peptide 2 ELISA Kit, CALCA ELISA Kit, CALCB ELISA Kit, ci-ct ELISA Kit, calca ELISA Kit, Calca ELISA Kit, LOC100008771 ELISA Kit
    Gene ID
    12310
    UniProt
    Q99JA0
    Pathways
    Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour
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