KNG1 ELISA Kit

Catalog No. ABIN5690746

Product Details KNG1 ELISA Kit

Target: KNG1 (Kininogen 1 (KNG1))

Reactivity: Human, Mouse, Rat

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0.1 ng/mL - 1000 ng/mL

Minimum Detection Limit: 0.1 ng/mL

Application: ELISA

Purpose: Human/Mouse/Rat Bradykinin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This EIA kit is designed to detect human, mouse, and rat Dynorphin

Sensitivity: 1.4 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Reagent Preparation:

Recommended Dilution for serum and plasma samplesHuman: sample extraction / Mouse: sample extraction / Rat: sample extraction

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Expiry Date: 6 months

Target Details for KNG1

Target: KNG1 (Kininogen 1 (KNG1))

Alternative Name: Bradykinin (KNG1 Products)

Synonyms: BDK ELISA Kit, BK ELISA Kit, KNG ELISA Kit, Kng ELISA Kit, KNG2 ELISA Kit, fb64g01 ELISA Kit, wu:fb64g01 ELISA Kit, zgc:103569 ELISA Kit, kininogen ELISA Kit, bdk ELISA Kit, kng ELISA Kit, KNG1 ELISA Kit, IHRP ELISA Kit, KINKG ELISA Kit, KINKH ELISA Kit, Kng1 ELISA Kit, Kngk ELISA Kit, kininogen 1 ELISA Kit, kininogen 1 L homeolog ELISA Kit, inter-alpha-trypsin inhibitor heavy chain family member 4 ELISA Kit, kininogen 2-like 1 ELISA Kit, KNG1 ELISA Kit, Kng1 ELISA Kit, kng1 ELISA Kit, kng1.L ELISA Kit, ITIH4 ELISA Kit, Kng2l1 ELISA Kit

Gene ID: 25087

UniProt: P08934

Pathways: ACE Inhibitor Pathway, Glycosaminoglycan Metabolic Process