SLC32A1 ELISA Kit

Catalog No. ABIN5690971

Product Details SLC32A1 ELISA Kit

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human VIAAT/SLC32A1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Human VIAAT.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 6 months

Target Details for SLC32A1

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Alternative Name: VIAAT (SLC32A1 (SLC32A1 Products)

Synonyms: VGAT ELISA Kit, VIAAT ELISA Kit, vGAT ELISA Kit, Ci-vGAT ELISA Kit, slc32a1 ELISA Kit, viaat ELISA Kit, MGC68938 ELISA Kit, vgat ELISA Kit, xVIAAT ELISA Kit, R75019 ELISA Kit, Viaat ELISA Kit, CG8394 ELISA Kit, CG8394.2 ELISA Kit, Dmel\\CG8394 ELISA Kit, Vgat ELISA Kit, zgc:158324 ELISA Kit, solute carrier family 32 member 1 ELISA Kit, vesicular GABA transporter ELISA Kit, solute carrier family 32 member 1 S homeolog ELISA Kit, vesicular inhibitory amino acid transporter ELISA Kit, Vesicular inhibitory amino acid transporter ELISA Kit, solute carrier family 32 (GABA vesicular transporter), member 1 ELISA Kit, Vesicular GABA Transporter ELISA Kit, SLC32A1 ELISA Kit, vgat ELISA Kit, Bm1_20950 ELISA Kit, slc32a1.S ELISA Kit, slc32a1 ELISA Kit, CpipJ_CPIJ002704 ELISA Kit, viaat ELISA Kit, Slc32a1 ELISA Kit, VGAT ELISA Kit, Tsp_04599 ELISA Kit

Gene ID: 140679

UniProt: Q9H598

Pathways: Proton Transport