Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

STAT2 ELISA Kit

STAT2 Reactivity: Human pTyr689 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate
Catalog No. ABIN5691025
  • Target See all STAT2 ELISA Kits
    STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))
    Binding Specificity
    pTyr689
    Reactivity
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-STAT2 (Tyr689) ELISA Kit. This assay semi-quantitatively measures STAT2 phosphorylated at Tyrosine-689 in cell lysate samples.
    Sample Type
    Cell Samples, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    This ELISA kit recognizes Human STAT2 phosphorylated at site Tyrosine-689.
    Characteristics
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Discover our best selling STAT2 ELISA Kit
    Top Product
    Discover our top product STAT2 ELISA Kit
  • Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all STAT2 ELISA Kits
    STAT2 (Signal Transducer and Activator of Transcription 2, 113kDa (STAT2))
    Alternative Name
    STAT2 (STAT2 Products)
    Synonyms
    wu:fj84d07 ELISA Kit, p113 ELISA Kit, stat2 ELISA Kit, 1600010G07Rik ELISA Kit, AW496480 ELISA Kit, ISGF-3 ELISA Kit, P113 ELISA Kit, STAT113 ELISA Kit, signal transducer and activator of transcription 2 ELISA Kit, AGAP000099-PA ELISA Kit, stat2 ELISA Kit, STAT2 ELISA Kit, Stat2 ELISA Kit
    Gene ID
    6773
    UniProt
    P52630
    Pathways
    JAK-STAT Signaling, Hepatitis C, CXCR4-mediated Signaling Events
You are here:
Support