ENPP2 ELISA Kit

Catalog No. ABIN5692213

Product Details ENPP2 ELISA Kit

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Binding Specificity: AA 36-863

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 625 pg/mL - 40000 pg/mL

Minimum Detection Limit: 625 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Autotaxin. 96wells/kit, with removable strips.

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Milk, Plasma (heparin), Serum, Urine

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO, Immunogen sequence: A36-I863

Sensitivity: < 10 pg/mL

Components: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Application Details

Assay Time: 15 min

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Expiry Date: 12 months

Target Details for ENPP2

Target: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Alternative Name: ENPP2 (ENPP2 Products)

Synonyms: ATX ELISA Kit, ATX-X ELISA Kit, AUTOTAXIN ELISA Kit, LysoPLD ELISA Kit, NPP2 ELISA Kit, PD-IALPHA ELISA Kit, PDNP2 ELISA Kit, Npps2 ELISA Kit, PD-Ialpha ELISA Kit, Pdnp2 ELISA Kit, MGC132047 ELISA Kit, atx ELISA Kit, zgc:63550 ELISA Kit, autotaxin ELISA Kit, enpp2 ELISA Kit, enpp2a ELISA Kit, enpp2b ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 L homeolog ELISA Kit, ectonucleotide pyrophosphatase/phosphodiesterase 2 S homeolog ELISA Kit, ENPP2 ELISA Kit, Enpp2 ELISA Kit, enpp2 ELISA Kit, enpp2.L ELISA Kit, enpp2.S ELISA Kit

Background:

Synonyms: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2

Tissue Specificity: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Background: Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene. The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.

Cellular Localisation: Secreted.

UniProt: Q13822