ATP1B1 ELISA Kit

Catalog No. ABIN5706895

Product Details ATP1B1 ELISA Kit

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: 0.066 ng/mL

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Sample Volume: 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12 months

Target Details for ATP1B1

Target: ATP1B1 (ATPase, Na+/K+ Transporting, beta 1 Polypeptide (ATP1B1))

Alternative Name: ATPase, Na+/K+ Transporting Beta 1 Polypeptide (ATP1b1) (ATP1B1 Products)

Synonyms: ATP1B ELISA Kit, Atp4b ELISA Kit, Atpb ELISA Kit, Atpb-1 ELISA Kit, NKbeta1 ELISA Kit, ATPBS ELISA Kit, atp1b1 ELISA Kit, atp1b1a ELISA Kit, cb710 ELISA Kit, ATPase Na+/K+ transporting subunit beta 1 ELISA Kit, ATPase, Na+/K+ transporting, beta 1 polypeptide ELISA Kit, ATPase beta chain ELISA Kit, ATP synthase CF1 beta subunit ELISA Kit, ATPase Na+/K+ transporting subunit beta 1 S homeolog ELISA Kit, ATPase, Na+/K+ transporting, beta 1b polypeptide ELISA Kit, ATP1B1 ELISA Kit, Atp1b1 ELISA Kit, atpB ELISA Kit, atp1b1.S ELISA Kit, atp1b1b ELISA Kit

Background: Alternative name: ATP1B, Sodium/Potassium-Dependent ATPase Subunit beta-1

Pathways: Thyroid Hormone Synthesis, Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome